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(The University of California, Riverside)

The University of California, Riverside, commonly known as UCR or UC Riverside, is a public research university and one of the ten general campuses of the University of California system. UCR is consistently ranked as one of the most ethnically and economically diverse universities in the United States.The main campus sits on 1,200 acres (486 ha) in a suburban district of Riverside, California, United States, with a branch campus of 20 acres (8 ha) in Palm Desert. Founded in 1907 as the UC Citrus Experiment Station, Riverside pioneered research in biological pest control and the use of growth regulators responsible for extending the citrus growing season in California from four to nine months. Some of the world's most important research collections on citrus diversity and entomology, as well as science fiction and photography, are located at Riverside.

More information: http://en.wikipedia.org/wiki/University_of_California,_Riverside

* Health Information Technology (HIT) California Council on Science and Technology February 2009 Alfonso F. Cardenas, UCLA
Teaching and California’s Future SRI International February 2, 2005

Teaching and California’s Future SRI International February 2, 2005

Title Page
A Report on the National Academy of Engineering and National Research Council Committee Two Year Study Rollie Otto, Panelist February 18 and 19 Sacramento California

A Report on the National Academy of Engineering and National Research Council Committee Two Year Study Rollie Otto, Panelist February 18 and 19 Sacramento California

Last revised 08Feb2009 RJO Edited GP Created RJO
Technology Needs In Telemedicine Rick Craft Lead, Telemedicine Reference Architecture Project Principal Member of the Technical Staff Sandia National Labs rlcraft@sandia.gov 505-844-8873

Technology Needs In Telemedicine Rick Craft Lead, Telemedicine Reference Architecture Project Principal Member of the Technical Staff Sandia National Labs rlcraft@sandia.gov 505-844-8873

PCAST Report: Priorities for Personalized Medicine
Health Care Information Technology Ramesh Rao California Institute for Telecommunication and Information technology University of California, San Diego

Health Care Information Technology Ramesh Rao California Institute for Telecommunication and Information technology University of California, San Diego

Office of Nuclear Energy, Nuclear Energy – Advanced Fuel Cycles and the Global Nuclear Energy Partnership Buzz Savage Office of Nuclear Energy U.S. Department of Energy Presentation to the California Council on Science and Technology May 24, 2006

Strategies to Reduce Transportation Oil Use and GHG Emissions Daniel Sperling UC Davis CCST May 24, 2006 Institute of Transportation Studies University of California, Davis Biofuels Plug-in’s FCV Hybrid Hydrogen Dan – Take a look at the agenda to get a handle on when and how you want to raise key issues in your two morning presentations. Labs and Fundraising for all things will be covered by me. Education overview will be covered by Pat. You need to decide what you really want to dwell on with the board to get their input on. I would suggest going through the “reporting/highlights” stuff fast first, then saying: “Okay, now I want to slow down and seek your ideas…”

Think globally, Assess regionally, Act locally Assessing the impacts of climate change in California

Developing Novel Supported Membrane Interfaces for SPR Study of Transmembrane Proteins Heather Ferguson*, Matthew J. Linman† , Quan Cheng† † Department of Chemistry - University of California Riverside 92521 *Walla Walla University, WA 99324 Introduction Membrane proteins are known key molecules in cellular activities such as maintaining cell structure, transport, and signalling. Therefore an understanding of the function, especially of the affinity property of membrane proteins, is of particular importance to the pharmaceutical industry where 60% of drug targets are membrane proteins.1 However, membrane proteins are difficult to study in their native environment and do not function properly when removed from a lipid membrane. We set out to mimic a mammalian lipid membrane environment using phosphatidylcholine (PC) vesicles that surround the transmembrane protein epidermal growth factor receptor (EGFR). EGFR is often overexpressed in cells in certain types of cancers such as breast, colon, and lung.2 Surface plasmon resonance (SPR) is employed to characterize interactions between the membrane protein and immobilized anti-EGFR TK, an antibody against the EGFR tyrosine kinase (TK) domain. Several surface chemistries have been examined in an effort to find a reproducible and functional biomimetic surface containing EGFR. Methods Results Conclusion Future Work OPTIONAL LOGO HERE References Acknowledgements Hopkins, A. L.; Groom, C. R. Nat.Rev. Drug Discovery 2002, 1...

Introduction This study deals with the effects of cholesterol on human embryonic kidney (HEK293) cells’ plasma membrane mechanics using an optical tweezers setup. Cholesterol regulates trans-membrane protein movement and plasma membrane-to-cytoskeleton attachment mechanics. Testing the effect of differing cholesterol concentrations on the biomechanics of HEK cells is integral to our understanding of the role cholesterol plays. Using the optical tweezers setup (Fig. 1), we extract nanotubes (tethers) from the plasma membrane under cholesterol-enriched and cholesterol-depleted conditions to determine the tether force. We are assuming that a second-order Maxwellian spring – dash plot model (Fig. 2) of viscoelasticity holds for the mechanics of these tethers2. Materials and Methods Static Force Calibration: The trapping force was calibrated by passing DMEM Complete (Dulbecco’s Modified Eagle Medium with FBS and Penicillin/Strep.) Serum-enriched through a trapped bead using a piezoelectric stage at various velocities (in µm/s) and measuring the velocity when the bead is dislodged from the trap. From these, we use a modified version of Stokes’ Law to calculate the Escaping Force (visible in Fig. 3). Static Tether Force Measurement: Using the Optical Tweezers setup, we brought a single cell in contact with a trapped bead and held the two together for a short time. After this time, the cell was moved away (at 1 µm/s) to pull a tether of ...

UNDERSTANDING DYNAMIC BEHAVIOR OF EMBRYONIC STEM CELL MITOSIS Shubham Debnath1, Bir Bhanu2 Embryonic stem cells are derived from the inner cell mass of early stage embryos, known as blastocysts. They are known to be pluripotent and can differentiate into a variety of different cell types, making them valuable for research for applications in medicine and healthcare. The Stem Cell Center at the University of California-Riverside has done previous and continuing research on the effects of cigarette smoke and alcohol on human and mouse embryonic stem cells. Video recordings taken over thirty-six hours show actions of cells under different conditions. The research aims to apply video processing techniques to characterize the processes of mitosis and pre-attachment behavior. Otsu’s algorithm is for binary thresholding and, when used, maximizes the variance between regions in a grey image. It can be applied in an iterative program to observe variations in cell to quantify several phenomenon. Software tools such as Matlab and ImageJ are used in this research. This presentation will include an overview of stem cells, different types and uses, and the importance in studying and understanding how stem cells interact with substrate and each other. A description of mitosis and attachment behaviors will precede an explanation of the approach of research, along with results, images, and normalized graphs. The graphs will display ways to quantify cellular process, including the...

Measuring Zeta Potential of M-Cells in Mucosal Epithelia Abstract Specialized microfold cells (M-cells) play an important role in transport of antigens across the mucosal lymphoid tissues to initiate an immune response. Understanding the mechanism by which M-cells bind antigens can provide insight to mucosal vaccination methods. Previous research has shown that surface charge of bacteria and potential delivery vehicles appears to play a significant role in M-cell selection. Thus, measuring electrostatic and zeta potential at the surface of shear between tissue and bacterial interaction remains as a main goal of our research studies. In this research, the streaming potential of M-cells cultured on membrane surfaces was determined. A streaming potential device was modified and tested to accommodate the membranes. The device was tested known samples consisting of Bovine Serum Albumin (BSA) adsorbed on composite regenerated cellulose (CRC) or polyethersulfone (PES) membranes with a molecular weight cutoff of 30 kDa. Then zeta potential was calculated by Helmholtz-Smoluchowski equation. Nilufer Nurinovaa, David Lob and Victor G. J. Rodgersb a Mathematics & CS Department, Tennessee Technological University b Bioengineering and Biomedical Sciences, University of California, Riverside Mucosal M-Cell Targeting Peyer’s Patch M-Cells Images courtesy of David Lo Membranes Used for Electrostatic Potential Test Composite Regenerated Cellulose Membrane (CRC) Surfa...

Development of Giant Unilamellar Vesicles for the Study of Crowded Protein Environments David D. Gooray, Sandeep Dhall, and Victor G. J. Rodgers Bioengineering Department, University of California, Riverside Giant unilamellar vesicles (GUVs) are supramolecular structures consisting of amphiphiles that range in sizes from 10-100 µm.[1] They provide an easy method to: (1) observe environment for in-vitro studies of compartmentalized reactions and (2) model particular cell behavior. By studying the effects of crowded protein environments within GUVs, we will achieve a greater understanding of processes and properties such as mitochondrial swelling and osmotic pressure. Phosphatidylcholine lipids are the only lipids that can form GUVs under electroformation.[2] GUVs with a diameter ranging between 50-100 µm will withstand micromanipulation techniques and allow us to study the effects of crowded protein environments. We prepared GUVs using the 3 types of phosphatidylcholine lipids; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), L-α-phosphatidylcholine (Egg PC) and 1-2-dioleoyl-sn-glyeo-3-phosphocholine(DPC). A square chamber was cut from teflon and molded to fit the microscope slide. Two cylindrical platinum wires (Sigma-Aldrich) of 1.0 mm diameter were housed approximately parallel to each other with a 3.0 mm separation Each wire was 1.5 mm from transparent viewing slide All drilled holes and platinum-teflon interfaces were sealed with silicone Preparation of...

Oxygen Reduction and Methanol Oxidation Reaction in Alkaline Methanol Fuel Cell Tam Duong, Toby Liu, Yan Yushan Ph.D Department of Chemical and Environmental Engineering Motivation: -Methanol is really easy to store because it is in liquid state at room temperature. That makes transportation becomes more effectively.. -Fuel cell can act like a battery that does not really go down or need to be charged if methanol and oxidizer continue to be supplied. Fuel cell can produce 0.6-0.7 V for a long period of time. Therefore, it is highly promising to serve as a power source of cell phones, or laptops. -Reducing pollution by reducing the amount of CO2. Introduction: -The cost of platinum which is the catalyst used for oxygen reduction reaction (ORR) and methanol oxidation reaction (MOR) in fuel cell activity is still really high. Other catalysts have been tested in acidic solution to substitute platinum; however, the better activities are found in alkaline solution. There are more choices of metals which can be used as catalysts in alkaline solution. With the Nafion membrane, many catalysts with different loadings have been used to test the ability of replacing platinum. -Although many catalysts work out for the oxygen reduction reaction and methanol oxidation reaction, they are not really able to replaced platinum. However, AgNW has given a promising result. Materials and Experiments: Table of Samples -System: three...

Genetic Incorporation of Unnatural Amino Acids into Proteins Monica Amin1, Yang Song2, Yan Liu2, Harbani Malik2, Vipul Madahar2, Jiayu Liao2 Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, New York Department of Bioengineering, University of California, Riverside Introduction This research was supported by the National Science Foundation Conclusion Results Deiters, Alexander, and Peter G. Schultz. "In vivo incorporation of an alkyne into proteins in Escherichia coli." Bioorganic & Medicinal Chemistry Letters (2005): 1521-524. Print. Liu, Wenshe, Ansgar Brock, Shuo Chen, and Peter G. Schultz. "Genetic Incorporation of Unnatural Amino Acids into Proteins in mammalian cells." Nature Methods 4.3 (2007): 239-44. Print. Martin, Sarah F., Michael H. Tatham, Ronald T. Hay, and Iford D.W. Samuel. "Quantitative analysis of multi-protein interactions using FRET: Application to the SUMO pathway." Protein Science (2008): 777-84. Print. Sapsford, Kim E., Lorenzo Berti, and Igor L. Medintz. "Materials for Fluorescence Resonance Energy Transfer Analysis: Beyond Traditional Donor- Acceptor Combinations." Angewandte Chemie (2006): 4562-588. Print. Wang, Lei, and Peter G. Schultz. "Expanding the genetic code." ChemCommun (2002): 1-11. Print. Zhang, Zhiwen, Brian A.C. Smith, Lei Wang, Ansgar Brock, Charles Cho, and Peter G. Schultz. "A New Strategy for the Site-Specific Modification of Proteins in Vivo." Biochemistry (2003): 6735-746. Print. Inco...

A genetically programmable protein module as intracellularly deliverable QD-based FRET probes for viral protease detection Nikola Finneran, Divya Sivaraman, Payal Biswas, and Wilfred Chen Department of Chemical and Environmental Engineering, University of California, Riverside, CA, 92521 Abstract Proteases are enzymes that are used in various cellular processes such as blood coagulation, hormone maturation and apoptosis. They are also used as the key virulence factor for pathogenic infection. These properties make proteases a prime target for detailed investigation to better understand the disease development process and can be potentially used to study various therapeutic agents. One of the most promising methods for probing protease activity is based on the principle of fluorescence resonance energy transfer (FRET). In this study, we develop a genetically programmable protein module that is easily adaptable for screening inhibitors for a wide range of proteases. The specific approach was to generate a quantum dot (QD)-modified, protease-specific protein module that can be used as a FRET substrate for probing protease activity. Intracellular delivery of the probes was facilitated by the use of a flanking TAT peptide and the site-specific incorporation of an acceptor fluorescent dye was accomplished using a unique cysteine residue. Presence of an elastin domain within the module enabled the simple purification of the QD-modified FRET substrate. For the initial...

Image Processing in Spectral Domain Optical Coherence Tomography (SD-OCT) Vasilios Morikis1,2, Dan DeLahunta1,3, Md. Shahidul Islam4, Christian M. Oh4, Hyle Park4 1 Bioengineering Research Institute for Technical Excellence, UC Riverside 2 Department of Nanotechnology, UC San Diego 3 Department of Physics, University of Rochester 4 Department of Bioengineering, UC Riverside Abstract Optical Coherence Tomography (OCT) is an optical imaging technique based on low coherence interferometry of light waves. This method is mostly useful for obtaining high resolution cross-sectional images of biological tissues at a high speed. OCT is advantageous for some of its features which include non-invasive procedures, minimal contact with tissues, use of non toxic dyes, good lateral and axial resolution of images and better in-depth imaging than other optical methods. Because of these features, OCT has been an important imaging method in Ophthalmology, Dermatology, Cardiovascular imaging, Neuroimaging and many other fields. An OCT system utilizes low coherent light source and an optical set up to produce interference patterns in the spectrometer and these interference patterns, termed as sample depth profiles, are later processed in the computer to obtain the final image of the sample. This project is looking at the post processing steps in an OCT system and the goal is to analyze the data obtained from the spectrometer and perform the image processing techniques to generate the fina...

Introduction The determination of protein-protein interaction affinity is very important in studying cell signaling pathways such as the SUMO pathway, and the best measurement of protein interaction is through the determination of the equilibrium binding constant Kd. Here we used an optical method based on steady-state Förster resonance energy transfer (FRET) to determine the Kd. FRET occurs over distances between 1-10nm. This is useful because FRET can be used to determine if proteins interact due to its distance dependence. 2 FRET also has many advantages over conventional techniques because protein concentrations, a vital part of equilibrium constant calculations, can be accurately determined through absorbance, and also small testing volumes can be used, allowing the assay to be developed into a multi-well plate assay. In our experiment we fused the fluorescent proteins CYPET and YPET with SUMO1 and UBC9, respectively. SUMO1 and UBC9 are known interacting proteins in the SUMO pathway, while CYPET and YPET are able to form a FRET pair with CYPET as the donor and YPET as the acceptor. Timothy Chen, Vipul Madahar, Yang Song, Dr. Jiayu Liao Department of Bioengineering, University of California, Riverside Department of Bioengineering, University of California, Berkeley Conclusion Our calculated value for Kd is in the same range as that calculated from the previous paper’s FRET experiment whose Kd = .59 μM.4 With this we have established that the use of...
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