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A total of 355 eligible cases (from 542 questionnaires) were identified which were not from an outbreak, not associated with foreign travel and had no other household members with diarrhoea. Permission was sought to follow up farm animal or environmental exposures.
The contact animal and environmental samples were analysed for the presence of Cryptosporidium oocysts by immunomagnetic separation and immunofluorescence microscopy (IFM), PCR-RFLP at the 18s rRNA locus, PCR at the dhfr locus and analysis of part of the GP60 gene. Sequencing was used to confirm speciation results and for GP60 subtyping. ZOONOTIC TRANSMISSION OF CRYPTOSPORIDIUM - A 2-YEAR EPIDEMIOLOGICAL STUDY Smith, R.P.1, Chalmers, R.M.3, Elwin, K.3, Hadfield, S.3, Mueller-Doblies, D.2, Clifton-Hadley, F.A.2, Watkins, J.4 and Giles, M.2 (1) Centre for Epidemiology and Risk Analysis (CERA), Veterinary Laboratories Agency Weybridge (VLA), New Haw, Addlestone, Surrey, KT15 3NB, United Kingdom
(2) Food and Environmental Safety (FES), Veterinary Laboratories Agency Weybridge (VLA), New Haw, Addlestone, Surrey, KT15 3NB, United Kingdom
(3) UK Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea, SA2 8QA, United Kingdom
(4) CREH Analytical, Leeds, LS18 4RS. Method:
Between Nov 2004 and Nov 2006, 28 diagnostic laboratories, in 41 local authorities within the study areas of South West and E...
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