Kinetic properties of Penicillium brasilianum cellulose degrading enzymesSTUDENT PROJECT at Cellulose is a chemically simple, but extremely recalcitrance biopolymer. It is composed of β-1,4-linked glucose chains tightly bound to each other. The basic 3D structure is called a microfibril, and consists of 40 glucan chains bound in a tight, water-excluding complex. Moreover, amorphous and crystalline regions within the cellulose chain can be distinguished.
In this study, two model substra
Kinetic properties of Penicillium brasilianum cellulose degrading enzymes
STUDENT PROJECT at Cellulose is a chemically simple, but extremely recalcitrance biopolymer. It is composed of β-1,4-linked glucose chains tightly bound to each other. The basic 3D structure is called a microfibril, and consists of 40 glucan chains bound in a tight, water-excluding complex. Moreover, amorphous and crystalline regions within the cellulose chain can be distinguished.
In this study, two model substrates – Avicel and Phosphoric Acid Swollen Cellulose (PASC) will be used to determine the kinetics of three cellulolytic enzymes. Substrate Lisbeth Olsson
Associate professor
lo@biocentrum.dtu.dk Enzymes The action of three classes of enzymes is needed to degrade cellulose: Endoglucanase Cellobiohydrolase β-glucosidase Cellobiose and shorter cellooligosaccharides (DP < 6) Substrate Mode of
action Product Surface of long cellulose microfibrils (primarily within amorphous regions) Reducing or non-reducing end “Endo”, random “Exo”, processive Cellobiose
(Strong inhibitor) Glucose
(Weak inhibitor) retaining Shorter hydrolyzed segments Symbol E1 E2 E3 Natalija Andersen
PhD student at the
Novozymes BioProcess Academy
na@biocentrum.dtu.dk Contact persons: Kinetics The aim of the study is to experimentally determine single enzyme kinetic constants - Km, kcat and Vmax under pre-determined hydrolysis conditions (T=50°C and pH=5.5). In practice, one determines initial rates for several s...
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