Nanoparticle mediated Delivery and Imaging
Sarah Fredriksson, PhD
Founder of Genovis AB I am sorry that I missed your meeting. Here I have put together a few slides presenting our nanoparticles. Hope to see you in the near future!
/Sarah Here I show a film were you can see the cell responding to a magnetic field. The cell is green since it has taken up millions of nanoparticles carrying fluorescent probes on their surfaces. I will attach the film separately.
Nanoparticle mediated Delivery and Imaging
Sarah Fredriksson, PhD
Founder of Genovis AB I am sorry that I missed your meeting. Here I have put together a few slides presenting our nanoparticles. Hope to see you in the near future!
/Sarah Here I show a film were you can see the cell responding to a magnetic field. The cell is green since it has taken up millions of nanoparticles carrying fluorescent probes on their surfaces. I will attach the film separately.
GENOVIS IN BRIEF:
Focus on Nanotechnology for Life Science
Patent pending technologies
Founded in 1999, R&D intense years 2000 – 2007
Starts marketing and building sales channels in 2008
Principal Business areas:
Biomolecule Delivery
Imaging
Facility in Lund, Sweden
9 employees
The Competitive Edge of Genovis Team Application driven design of unique nanostructures for use in (combined) delivery and imaging applications
Production skills
Products on the market for in vitro use
Strong technical and practical customer support
Pipeline with focus on in vivo imaging and drug delivery
Diagnostics
Drug delivery
Imaging
Novel therapeutics NANOPARTICLE APPLICATIONS IN BIOMEDICINE
Nanoparticles
Nanoparticles in relation to micro
The surface to volume ratio increases with a factor of 1000
The material characteristics as we know them can change
Production processes become a challenge
Nanoparticle structures (< 300 nm)
Shell structures
Solid core particles
Polymer based formulations
Lipid based formulations
and more …..
Genovis has developed NIMT FeOdots – nanoparticles for Life Science Applications
Hi,
I am a FeOdot. NIMT FeOdots are superparamagnetic nanoparticles specially designed for use in the fields of biomedicine and biotechnology. FeOdots are engineered nanoparticles of solid iron oxide cores composed mainly of Fe3O4 (Magnetite). Due to non classical synthesis routes size and shape can be controlled. The nanoparticles have a high magnetic saturation (Ms) of about 80 emu/g and are monodispersed (11 nm ± 1 nm core size).
Solid homogenous iron oxide cores
Spherical shape
Possibility to vary core diameter Superparamagnetic iron oxide cores
Superparamagnetism = no ”magnetic memory” when M-field is removed Particle cores < approx. 20 nm generally exhibits superparamagnetic behaviour
Particle coating objectives:
Surface for binding/protecting the cargo molecule (DNA/siRNA/peptide
etc) during transport
Cargo release
Suitable for bioconjugation
Promote colloidal stability
Possibility of fluorescence
Prevent unspecific interactions Particle coating
Particles designed from inside and out:
Two coating procedures were developed: PEG based & Lipid based Iron Oxide H2N NH2 PEG Phospholipid Phospholipid-Texas Red Iron Oxide H2N NH2 FeOfection|PURPLE FeOfection|YELLOW FeOfection|PINK
Fig 1. Particles incubated for 48 hours in different pH.
Even under extreme salt concentrations (Fig 2) the particles remain in solution with no observed agglomeration. This has been further confirmed by size measurements using dynamic light scattering (DLS).
Fig 3. NIMT FeOdots incubated with 1-5 M NaCl for 48 hours.
The extreme stability of NIMT FeOdots are of importance for in vivo applications or application were complex medium is used. The magnetic nanoparticles remain stable in serum and other high protein concentration solutions for a extended period of time. No agglomeration or precipitations are observed upon incubation in human serum (Fig 4). This has been further confirmed by size measurements using dynamic light scattering (DLS).
Fig 2 Colloidal stability is important
Colloidal stability of PEG-Biotin particles
Nanoparticles in combined delivery and imaging Spinning Endosomes – this is a film clip showing how the interior of each endosome can follow an external magnetic field. The endosomes have taken up nanoparticles carrying a flourescent probe on their surface. I am not sure whether I can attach the film in the e-mail. Otherwise I hope that I will get an opportunity later. Cell nucleus
siRNA delivery –product application
The nanoparticles consists of a stable iron-oxide core
By varying the lipids and coatings of the particles the particles can be tailor-made to promote uptake and release of siRNA in different cell lines.
FeOfection| is coated with cationic lipids to facilitate siRNA binding and cellular uptake
siRNA Delivery Using NIMT FeOfection
Gene Silencing w/ NIMT®FeOfectionPURPLE
HEK cells transfected with Eg5 siRNA
Cells lacking Eg5 cannot divide and therefore do not stay in their rounded up stage. Cell growth is arrested and the cells eventually die.
A.
B. siRNA Eg5 treated cells. 48 h post transfection siRNA negative control treated cells. 48 h post transfection
Simplify - siRNA delivery – NIMT® FeOfection
Gene Delivery Att tillföra en ny gen i en cell FeOfection is a solution of nanoparticles with an iron oxide core.
The core is stable and the magnetic properties can be used e.g. in tracking of cells with MRI.
The surface of the particles are modified to promote binding of DNA to the particles and facilitate transport of the resulting particle/DNA complexes into cells.
FeOfection can be used for both transient (temporary expression) and stable (incorporated in the genome) transfection.
Imaging using magnetic nanoparticles Marknaden drivs av ett medicinskt behov av effektivare och känsligare diagnostik
Iron Oxide NH2 Phospholipid Amino-PEG NHS-Alexa 647 Iron Oxide NH2 U-2 OS cell incubated with Alexa-647 magnetic nanoparticles for 1 hour FeOdots incubated with cells and exposed to a magnetic field NH2 NH2
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